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The paperpresents the results of studies to determine the fractional composition of biofuels, bio-component containing the FAME and diesel. Formulated biofuels B containing 7%, 15%, 25%, 50% and 100% of FAME. Biofuels are produced from FAME use oil in the reactor of his own design type GW 200. The study was conducted in accordance with the requirements of BS EN ISO 3405:2012 (ASTM D 1160) using automated distillers HAD 620/1 by Herzog. Experiments have shown that both the beginning of distillation and its course depending on the amount of RME in diesel fuel. The greater the amount of FAME biodiesel that later starts the beginning of the distillation. Experiments have shown that both the beginning of distillation and its course depending on the amount of FAME in diesel fuel. The greater the amount of FAME biodiesel that later starts the beginning of the distillation. Among the studied biofuels only B7 and B15 FAME can meet the requirements for a standard diesel, which states that 95% v/v fuel must evaporate to a temperature of 360C (633 K).