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For several years, research is underway to fuels derived from natural sources, which can partially or completely replace the fuel from crude oil. Such fuels are called non-conventional, alternative or substitute. As a substitute fuels commonly used in CI engines can provide liquid fuels of vegetable origin or another, and gaseous fuels. In the past decade, used vegetable oil methyl esters (FAME), Poland - rapeseed oil esters. Currently, notes the trend of so-called withdrawal biodiesel, including the opinion of the speaker because of the negative effect of that not only the fuel injection equipment, as well as CI engine performance. This paper presents the results of the influence the content of FAME (vegetable oil methyl esters) in diesel oil for power, torque, and the content of harmful substances in exhaust gases CI engine. These results were related to the obtained with the engine running only on pure diesel oil. The results were obtained on test bed for engine and for car.