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The article presents a comparative study of carbon monoxide, hydrocarbons, nitrogen oxides and the mass and number of particulate of diesel engine with the power diesel and fuel B100. B100 fuel is a biofuel produced based on vegetable oils for vehicles with compression-ignition engines. B100 fuel and diesel have similar physical-chemical characteristics of which have been analyzed. The research was carried out on an engine dynamometer in four cycles: ESC, ETC and WHTC and WHSC. The article was carried out analysis of the results of research in this regard, preceded by a discussion of the test cycles used.