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The article presents results on combustion of the bio-oil blended butanol in the spark ignition engine. Bio-oil is a mixture of hydro-carbons condensing to liquified phase while cooling it down to ambient temperature. In general, the liquid called bio-oil is a byproduct of the pyrolysis process of organic matter. Results from analysis presented in the manuscript include the following: in-cylinder pressure traces and toxic exhaust emissions. Finally, comparison of these results with results from combustion of n-butanol reference fuel were provided. Obtained results indicate satisfactory, eco-friendly possibility for utilization of bio-oil in the internal combustion engine