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The aim of the study was to determine how using the 2^nd generation biodiesels generated from the fat obtained from the leather industry as additives affects the fractional composition of fuels. The works included testing biodiesels with the five-, twenty-, fifty-percent content (v/v) of the 2^nd generation biocomponents, i.e. B5, B20 and B50 fuels were generated. The results of a study of pure diesel fuel and the 1^st generation RME biodiesel were used for comparison. The study has shown that the 2^nd generation biodiesel has better distillation properties than the 1^st generation RME biodiesel. The biofuels containing the 2^nd generation biocomponent generally also had a better fractional composition than the fuels containing the 1^st generation RME biocomponent. The 1^st and 2^nd generation biodiesels were produced using a GW-200 self-made reactor (by Grzegorz Wcisło).